Journal: Cell Death Discovery

Article Title: c-Myb protects cochlear hair cells from cisplatin-induced damage via the PI3K/Akt signaling pathway

doi: 10.1038/s41420-022-00879-9

Figure Lengend Snippet: A Western blot showed a significantly decreased ratio of P-Akt/Akt and decreased level of P-PI3K (p85α) in the cultured HCs treated with cisplatin + AAV-ie for 48 h. The levels of P-PI3K (p85α) and P-Akt were significantly reduced in HCs co-treated with cisplatin, AAV-ie and LY294002 compared to the cisplatin + AAV-ie group, while they were significantly increased in HCs co-treated with AAV-c-Myb and cisplatin. The treatment with LY294002 decreased the above effects of c-Myb on p85α level and the ratio of P-Akt/Akt in the cisplatin + AAV-c-Myb + LY294002 group compared to the cisplatin + AAV-c-Myb group. B – F Immunostaining and cell counting showed that the overexpression of c-Myb reduced HC loss after cisplatin injury, but the treatment with LY294002 significantly increased the HC loss in the cisplatin + AAV-c-Myb + LY294002 group compared to the cisplatin + AAV-c-Myb group. Scale bar = 20 μm. *** p < 0.001, n = 6 for each group.

Article Snippet: The primary antibodies were as follows: anti-c-Myb antibody (1:1000 dilution, Millipore, USA), anti-P-Akt (1:1000 dilution; Cell Signaling Technology), anti-Akt (1:1000 dilution; Cell Signaling Technology), anti-P-PI3K (1:1000 dilution; Cell Signaling Technology), and anti-β-actin (1:2000 dilution; ZSGB-BIO).

Techniques: Western Blot, Cell Culture, Immunostaining, Cell Counting, Over Expression

Journal: British Journal of Cancer

Article Title: Phosphorylation of the androgen receptor is associated with reduced survival in hormone-refractory prostate cancer patients

doi: 10.1038/sj.bjc.6604152

Figure Lengend Snippet: Histoscore variation and comparison of staining intensity for hormone-sensitive and hormone-refractory tumours

Article Snippet: Phosphatidylinositol 3-OH kinase (Cell Signalling Technology, Beverly, MA, USA), Akt1–3 (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), pAkt 473 (Cell Signalling Technology), mTOR (Santa Cruz Biotechnology Inc.), pmTOR 2448 (Cell Signalling Technology) and pAR 210 (Imgenex, San Diego, CA, USA) antibodies were used at the following concentrations (1, 1, 2, 2, 4, 5, 2 and 50 μ g ml −1 ).

Techniques: Staining

Journal: British Journal of Cancer

Article Title: Phosphorylation of the androgen receptor is associated with reduced survival in hormone-refractory prostate cancer patients

doi: 10.1038/sj.bjc.6604152

Figure Lengend Snippet: ( A ) Kaplan–Meier plot demonstrates that those patients whose tumours exhibit a rise in PI3K expression (broken line) relapse quicker than those patients whose tumours exhibit no change or a fall in PI3K expression (solid line). ( B ) Kaplan–Meier plot demonstrates that those patients whose tumours exhibit a rise in pAR 210 expression (broken line) have shorter time to disease-specific death from time of biochemical relapse than those patients whose tumours exhibit no change or a fall in pAR 210 expression (solid line). ( C ) Kaplan–Meier plot demonstrates that those patients whose tumours exhibit a rise in pAkt 473 cytoplasmic expression (broken line) have shorter time to disease-specific death than those patients whose tumours exhibit no change or a fall in pAkt 473 expression (solid line). ( D ) Kaplan–Meier plot demonstrates that those patients whose tumours exhibit a rise in pAR 210 expression (broken line) have shorter time to disease-specific death than those patients whose tumours exhibit no change or a fall in pAR 210 expression (solid line).

Article Snippet: Phosphatidylinositol 3-OH kinase (Cell Signalling Technology, Beverly, MA, USA), Akt1–3 (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), pAkt 473 (Cell Signalling Technology), mTOR (Santa Cruz Biotechnology Inc.), pmTOR 2448 (Cell Signalling Technology) and pAR 210 (Imgenex, San Diego, CA, USA) antibodies were used at the following concentrations (1, 1, 2, 2, 4, 5, 2 and 50 μ g ml −1 ).

Techniques: Expressing

Journal: British Journal of Cancer

Article Title: Phosphorylation of the androgen receptor is associated with reduced survival in hormone-refractory prostate cancer patients

doi: 10.1038/sj.bjc.6604152

Figure Lengend Snippet: Scatter plots of pAkt 473 histoscore compared to pAR 210 histoscore, ( A ) is in the hormone-sensitive tissue and no significant correlation was observed ( P =0.061, correlation coefficient 0.251); however, ( B ) is in the hormone-refractory tissue, where a significant correlation was observed ( P <0.001 and correlation coefficient 0.711).

Article Snippet: Phosphatidylinositol 3-OH kinase (Cell Signalling Technology, Beverly, MA, USA), Akt1–3 (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), pAkt 473 (Cell Signalling Technology), mTOR (Santa Cruz Biotechnology Inc.), pmTOR 2448 (Cell Signalling Technology) and pAR 210 (Imgenex, San Diego, CA, USA) antibodies were used at the following concentrations (1, 1, 2, 2, 4, 5, 2 and 50 μ g ml −1 ).

Techniques: